Journal: Scientific Reports

Article Title: Overexpression of HSPA1A enhances the osteogenic differentiation of bone marrow mesenchymal stem cells via activation of the Wnt/β-catenin signaling pathway

doi: 10.1038/srep27622

Figure Lengend Snippet: ( A ) The endogenous expression of HSPA1A mRNA was determined by qPCR at days 0, 7, and 14 of osteogenic differentiation. ( B,C ) The endogenous expression of HSPA1A protein was determined by Western blot analysis at days 0, 7, and 14 of osteogenic differentiation. ( D ) rBMSCs after lentiviral transfection and puromycin screening were observed under a normal microscope and a fluorescence microscope. ( E ) The proliferation rate of rBMSCs was not significantly affected by HSPA1A overexpression. ( F,G ) Protein levels of HSPA1A were determined by Western blot analysis among the lenti- HSPA1A , mock treated group, and lenti-control group. The mRNA expression levels were normalized to that of 18S ribosomal RNA. The data are expressed as means ± standard deviation, n = 3, * P < 0.05 vs. the lenti-control group.

Article Snippet: Lentivirus overexpressing HSPA1A (lenti- HSPA1A , pLV[Exp]-Puro-CMV >rat- HSPA1A [NM_031971.2]: IRES: EGFP) particles and lentiviral GFP particles (lenti-control, pLV[Exp]-Puro-CMV >EGFP) were prepared by Cyagen Biosciences.

Techniques: Expressing, Western Blot, Transfection, Microscopy, Fluorescence, Over Expression, Control, Standard Deviation